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J. Biochem, 1982, Vol. 92, No. 3 725-736
© 1982 Japanese Biochemical Society

research-article

Isolation and Characterization of the Golgi Complex from Rat Ascites Hepatoma AH-130 Cells1

Keikichi TAKAHASHI2, Yukio IKEHARA3 and Keitaro KATO

Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University 62 Higashi-ku, Fukuoka, Fukuoka 812

3 To whom all correspondence should be addressed

A Golgi-rich fraction has been isolated from rat ascites hepatoma AH-130 cells. Unlike the usual procedure for isolating Golgi complexes from liver and other tissues, a hypotonic solution including 2 mM CaCl2 was used as the homogenization medium for the ascites hepatoma cells, followed by a combination of differential and discontinuous sucrose gradient centrifugations.

Electron microscopic observation revealed that the isolated fraction consisted of cisternae, vesicles and tubular elements which were similar to those structures described previously for the Golgi fraction isolated from rat liver. Galactosyl- and sialyl-transferases were concentrated about 55- and 75-fold, respectively, in this fraction compared with the homogenate, indicating that these enzymes are useful markers for the Golgi complex of rat ascites hepatoma AH-130 cells, as they are for those of other normal tissues. The preparation was virtually free of cytochrome oxidase, but contained minor amounts of acid phosphatase, alkaline phosphatase and phosphodiesterase I activities.

Electrophoretic analysis on sodium dodecylsulfate-polyacrylamide gels showed that the hepatoma Golgi membranes were resolved into at least 23 protein bands, which were apparently different from the electrophoretic profile of the plasma membrane isolated from the same hepatoma cells.

1 This work was supported in part by Grants-in-Aid Scientific Research from the Ministry of Education, Science and Culture of Japan, and by a grant from the Fukuoka Anti-Cancer Association.

2 Present address: Cancer Research Institute, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka, Fukuoka 812.

Present address: Department of Biochemistry, Fukuoka University School of Medicine, 34 Nanakuma, Jonan-ku, Fukuoka, Fukuoka 814–01


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